Preparation and evaluation of silica-based ionic liquid-modified stationary phase for HPLC.

نویسندگان

  • Ye Wang
  • Tao Zhu
  • Kyung Ho Row
چکیده

A new high-performance liquid chromatography (HPLC) stationary phase was firstly synthesized based on the ionic liquid 2-ethyl-4-methylimidazole chloride. And the performances of the resulting stationary phase were determined by Fourier transform infrared (FT-IR), thermogravimetric analysis (TGA), and (13)C nuclear magnetic resonance (NMR) spectroscopy. The retention characteristics of the resulting stationary phase were evaluated by normal phase HPLC conditions to separate tanshinone I and tanshinone IIA in Danshen herb. The results demonstrated that the synthesized ionic liquid was bond-linked to the silica surface successfully and that IL-modified silica is more effective than unmodified silica when separating tanshinone I and tanshinone IIA in Danshen herb.

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عنوان ژورنال:
  • Journal of chromatographic science

دوره 48 8  شماره 

صفحات  -

تاریخ انتشار 2010